RNAi Screening: Automated High-Throughput Liquid RNAi Screening in Caenorhabditis elegans

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

High-throughput RNAi screening for germline apoptosis genes in Caenorhabditis elegans.

Among the greatest tools that Caenorhabditis elegans can provide researchers are the capabilities to perform high-throughput, genome-wide screens. Using bacterial RNAi libraries, which cover the majority (>85%) of the worm genome, genes can be rapidly and systematically evaluated for apoptosis phenotypes in the germline. Screens can be designed to directly assess the levels of apoptotic corpses...

متن کامل

Automated microscopy for high-content RNAi screening

Fluorescence microscopy is one of the most powerful tools to investigate complex cellular processes such as cell division, cell motility, or intracellular trafficking. The availability of RNA interference (RNAi) technology and automated microscopy has opened the possibility to perform cellular imaging in functional genomics and other large-scale applications. Although imaging often dramatically...

متن کامل

High-throughput RNAi screening reveals novel regulators of telomerase.

Telomerase is considered an attractive anticancer target on the basis of its common and specific activation in most human cancers. While direct telomerase inhibition is being explored as a therapeutic strategy, alternative strategies to target regulators of telomerase that could disrupt telomere maintenance and cancer cell proliferation are not yet available. Here, we report the findings of a h...

متن کامل

SbacHTS: Spatial background noise correction for High-Throughput RNAi Screening

MOTIVATION High-throughput cell-based phenotypic screening has become an increasingly important technology for discovering new drug targets and assigning gene functions. Such experiments use hundreds of 96-well or 384-well plates, to cover whole-genome RNAi collections and/or chemical compound files, and often collect measurements that are sensitive to spatial background noise whose patterns ca...

متن کامل

Realizing the promise of RNAi high throughput screening.

Recently reporting in Nature, Collinet et al. describes the application of quantitative multiparametric methods to a genome-wide RNAi screen for regulators of endocytosis. The study illustrates the power of this approach beyond the identification of new endocytic components to providing insights into the design principles of the endocytic system.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Current Protocols in Molecular Biology

سال: 2018

ISSN: 1934-3639

DOI: 10.1002/cpmb.65